Figure 3. DLX6-AS1 silencing inhibited neuronal apoptosis induced by brain ischemia/reperfusion. (A) Representative images and statistics of TUNEL staining of brain sections for assaying neuronal apoptosis. (B) Cleaved caspase-3 protein expression, measured by western blotting. Data are given as the mean ± SEM (n = 6 in each group). ***P < 0.001 vs sham; ++P < 0.01, +++P <0.001 vs IS 1 h/R 24 h + LV-NEG-shRNA.