Research Paper Volume 12, Issue 19 pp 19785—19808

V. unguiculata extract reduces α-synuclein toxicity in neuroblastoma cells. (A, B) SH-SY5Y cells were grown for 48 h in the absence (cnt) or presence of 5 μM α-synuclein solution obtained after 24 h (oligomers, Ol) and 72 h (fibrills, Fib) of aggregation, without or with extract at molar ratio protein:extract 1:0.5 (E0.5) and 1:1 (E1). (A) Cell viability assessed by MTT assay and (B) ROS level evaluated by DCFDA fluorescence intensity assays. *p<0.05; **p<0.01; ***p<0.001 vs untreated cells. °p<0.05; °°p<0.01 vs treated cells with α-synuclein aggregates oligomeric (Ol) and fibrillar (Fib) grown without extract. (C) Z-projection of SH-SY5Y cell images by α-synuclein immunostaining (red) and CTX-B plasma membrane staining (green). Scale bars are shown.