Research Paper Volume 12, Issue 20 pp 20801—20816
Synergistic effect between LH and estrogen in the acceleration of cumulus expansion via GPR30 and EGFR pathways
The activity and quantity of lysosomes were both downregulated by EGF treatment in FGCs. Lysosomal activity was detected by Lyso-Tracker red, bar=50 μm (A). The quantity of lysosomes was evaluated by immunostaining (B), GPR30 antibody (red), LAMP1 antibody (green), and DAPI (blue), shown in this panel, bar=10 μm. “Neg” indicates negative control (secondary antibodies only). Three independent replicates were performed for each experiment.