Research Paper Volume 12, Issue 23 pp 23739—23760

Figure 10. Effects of SDV on pseudo germ-free mice transplanted with fecal microbiota. (A) Treatment schedule. Mice underwent a splenectomy 28 days before FMT. After 14 days, the mice were given large doses of antibiotics for 14 days. Then, the mice were orally administered fecal microbiota from 20 mg/kg LPS-treated septic mice that had been exposed to light for three days. On days 15 and 18, behavioral tests were performed. Subsequently, the mice were euthanized, and their spleens were collected and weighed. (B) Pseudo germ-free mice that underwent SDV exhibited no significant differences in spleen weight after being transplanted with fecal microbiota from different groups. The mice also had similar behavioral performances, as evidenced by the lack of significant difference in the frequency of entering the novel arm (C) and the time spent in the novel arm (D) in the Y maze test, the time spent in the center (E) and the freezing time (F) in the open field test, and the time exploring the novel object (G, H) in the NORT. Data are shown as the mean ± SEM (n = 6/group). N.S., not significant.