Research Paper Volume 12, Issue 20 pp 20523—20539

Exosomal UCA1 participated in intercellular communication. (A) Expression of UCA1 in the medium of NEEC, EC18, and Kyse140 cells treated with RNase (2 ug/ml) or the combination of RNase (2 ug/ml) and Triton X-100 (0.1%). (B) Morphology and size of exosomes derived from the medium of NEEC, EC18, and Kyse140 cells, respectively. Scale bar = 100 nm. (C) Protein expressions of exosomal markers TSG101 and CD63 in exosomes derived from the medium of NEEC, EC18, and Kyse140 cells, respectively. (D) Relative expressions of UCA1 in exosomes and exosome-releasing cells. (E) Relative expressions of UCA1 in exosomes derived from NEEC, EC18, and Kyse140 cells. (F) Exosomes derived from NEEC cells were taken up by EC18, and Kyse140 cells. Exosomes were labeled with PKH67 (green), and the nuclei were labeled with DAPI (blue). Scale bar = 25 nm. (*) denotes the difference between groups (P<0.05). Each experiment had a minimum of three replicates per treatment group.