Figure 4. NEAT1 was upregulated in melanoma cells. (A) Venn diagram of intersection of target lncRNAs predicted by bioinformatics analysis. (B) RT-qPCR was performed to measure the level of NEAT1 in benign naevus (n=18) and melanoma tissues (n=18). (C) RNAscope detection of NEAT1 expression in melanoma tissues and benign naevus. Scale bars: 100 μm. (D) Quantitative analysis of NEAT1 expression in melanoma tissues (n=18) and benign naevus (n=18). (E) RT-qPCR was performed to measure the level of NEAT1 in various melanoma cells (A375, A875 and M14) and non-cancer cells, HEMa-LP.