Research Paper Volume 12, Issue 18 pp 18522—18544
GPA peptide inhibits NLRP3 inflammasome activation to ameliorate colitis through AMPK pathway
Figure 3. GPA inhibited NLRP3 inflammasome assembly and GSDMD cleavage. THP-1 cells were primed with LPS for 4h, followed by GPA treatment 6 h before stimulation with ATP for 30 min. Immunoblot analyzed of ASC oligomerization in lysates of THP-1 cells (A). IP and immunoblot analyzed of the interaction of endogenous NLRP3 and ASC in THP-1 cells (B). Immunoblot analyzed of NLRP3 by SDD-AGE assay in THP-1 cells (C). Immunoblot analyzed of cleavage of GSDMD in THP-1 cells (D). IP and immunoblot analyzed of the interaction of endogenous NLRP3 and GSDMD in THP-1 cells (E). Three independent experiments.