D-galactose induces senescence of glioblastoma cells through YAP-CDK6 pathway

Xingxing Xu; Xiya Shen; Wenjin Feng; Danlu Yang; Lingting Jin; Jiaojiao Wang; Mianxian Wang; Zhang Ting; Feng Xue; Jingjing Zhang; Chaobo Meng; Roumeng Chen; Xinru Zheng; Leilei Du; Lina Xuan; Ying Wang; Tian Xie; Zhihui Huang

doi:doi:10.18632/aging.103819

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Research Paper Volume 12, Issue 18 pp 18501—18521

D-galactose induces senescence of glioblastoma cells through YAP-CDK6 pathway

Figure 6. D-gal induced senescence of GBM cells by inactivating YAP-CDK6 pathway in a Hippo pathway dependent manner. (A) Western blot detected the protein expression of p-YAP, YAP, p-MST1, MST1, p-LATS1, LATS1, CDK6, p27^kip1, and Lamin B1 in control and senescent C6 cells (treated with 222 mM D-gal for 8 d). (B–I) Quantification of protein levels of p-YAP/YAP, YAP, p-MST1/MST1, MST1, p-LATS1/LATS1, LATS1, CDK6 and p27^kip1 (n=3) level as shown in (A). Data shown are mean ± s.e.m. ^*P < 0.05, ^**P < 0.01.