Research Paper Volume 12, Issue 19 pp 19352—19364

Identification of the targeting relationship between miR-197-5p and CHD9 in gastric cancer cells. (A) The binding sites of miR-197-5p and mutated CHD9-3’UTR were predicted by StarBase v.3.0. (B) The CHD9 expression status in different cell were shown. (C, D) The luciferase activity in SGC-7901 and MGC-803 cells co-transfected with CHD9-WT or CHD9-MUT and miR-197-5p-mimics or mimics-NC was evaluated by dual-luciferase reporter assay. (E, F) The mRNA and protein expression levels of CHD9 in SGC-7901 and MGC-803 cells transfected with mimics-NC, miR-197-5p-mimics, inhibitor-NC or miR-197-5p-inhibitor were examined by RT-qPCR and western blot, respectively. (G) CHD9 expression in gastric cancer tissues compared with in normal tissues was detected by RT-qPCR. (H) The correlation between miR-197-5p expression and CHD9 expression in gastric cancer tissues was analyzed by Pearson’s test (R²=0.6, P < 0.001). (I) The proliferation of SGC-7901 and MGC-803 cells transfected with mimics-NC, miR-197-5p-mimics, miR-197-5p-mimics + pcDNA3.1 or miR-197-5p-mimics + pcDNA3.1-CHD9 was examined by MTT assay. (J) The migration of SGC-7901 and MGC-803 cells was evaluated by Transwell assay without Matrigel; *** P < 0.001.