Research Paper Volume 12, Issue 19 pp 19352—19364

Studies on the relationship between circPDZD8 and miR-197-5p in gastric cancer cells. (A) The binding sites and mutated sites of circPDZD8 and miR-197-5p were predicted by StarBase v.3.0. (B, C) The luciferase activity in SGC-7901 and MGC-803 cells co-transfected with circPDZD8-WT or circPDZD8-Mut and miR-197-5p-mimics or mimics-NC was assessed by dual-luciferase reporter assay. (D) circPDZD8 expression level in SGC-7901 and MGC-803 cells transfected with miR-197-5p inhibitors or NC were measured by RT-qPCR. (E, F) miR-197-5p expression in gastric cancer tissues and cell lines was measured by RT-qPCR. (G) The correlation of circPDZD8 and miR-197-5p in gastric cancer tissues was analyzed by Pearson’s test (R²=0.6, P < 0.001). (H) The proliferation of SGC-7901 and MGC-803 cells transfected with si-NC, si-circPDZD8-1, si-circPDZD8-1 + inhibitor-NC or si-circPDZD8-1 + miR-197-5p-inhibitor was examined by MTT assay. (I) The migration of SGC-7901 and MGC-803 cells transfected with si-NC, si-circPDZD8-1, si-circPDZD8-1 + inhibitor-NC or si-circPDZD8-1 + miR-197-5p-inhibitor was detected by Transwell assay without Matrigel; *** P < 0.001.