Research Paper Volume 12, Issue 22 pp 22550—22563

Figure 2. XL388 induces significant apoptosis activation in glioma cells. A172 cells (A–F), U251MG (“U251”) (G and H) and primary human glioma cells (“Pri-1/Pri-2”) (G and H) as well as the primary human astrocytes (“Astrocytes”) and HCN-1a neuronal cells (I and J) were treated with XL388 (250 nM), and cultured for applied time periods, then cell apoptosis was analyzed by the mentioned assays (A–H and J), with cell viability tested by CCK-8 assay (I). Data were presented as mean ± SD (n=5). * p <0.05 vs. “C” cells. Experiments in this figure were repeated three times, and similar results were obtained. Bar= 100 μm (D and E).