Research Paper Volume 12, Issue 19 pp 19293—19315
Modification of Mcl-1 alternative splicing induces apoptosis and suppresses tumor proliferation in gastric cancer
The myeloid cell leukemia (Mcl)-1 splicing pattern shifts efficiently from Mcl-1L to Mcl-1S after delivery of the steric-blocking oligonucleotides (SBOs) into the gastric cancer (GC) cell lines. (A) Mcl-1L and Mcl-1S messenger RNA (mRNA) expression levels in GC cell lines after treatment with SBOs at different dosages are shown. Data of at least three independent experiments are shown as the means ± standard deviation. (B) Western blot showing the Mcl-1L and Mcl-1S protein levels in GC cell lines treated with phosphate-buffered saline or SBOs at the indicated concentrations is presented. This experiment was repeated thrice.