Research Paper Volume 12, Issue 19 pp 19233—19253

MiR-7-5p affects the biological characteristics by targeting KLF4 in HB cells. (A) Schematic illustration showing the overlapping possible target genes of miR-7-5p predicted by miRanda, PicTar, TargetScan, and miRmap; (B) Sequence alignment of CDR1as and KLF4 with miR-7-5p; (C) The relative luciferase activities were analyzed in HEK 293T cells that were co-transfected with miR-7-5p mimics or miR-mimics-NC and KLF4 wild type or Mut luciferase reporter vectors; (D) qRT-PCR analysis of the expression of KLF4 and miR-7-5p in HB cells that were treated with si-CDR1as; (E) qRT-PCR analysis of KLF4 mRNA in HB cells that were treated with miR-7-5p mimics; (F) Western blot analysis indicated that miR-7-5p downregulated KLF4, and this was similar to the effect of CDR1as siRNA on KLF4. Data are presented as the mean ± SEM of three experiments. **P < 0.01 (Student’s t-test).