Research Paper Volume 12, Issue 19 pp 19233—19253
CircRNA CDR1as promotes hepatoblastoma proliferation and stemness by acting as a miR-7-5p sponge to upregulate KLF4 expression
CDR1as affects the proliferation and stemness of HB cells. (A) qRT-PCR analysis of CDR1as in HB cells treated with siRNAs; (B) qRT-PCR analysis of CDR1 mRNA in HB cells treated with siRNAs; (C–E) Cell proliferation was assessed using the Cell Counting Kit-8 (CCK-8), colony formation assay, and EdU assay after knocking down CDR1as in HepG2 and HUH6 cells; (F) The stemness of the CSCs was assessed by the sphere-forming assay after knocking down CDR1as in HepG2 and HUH6 cells; (G) The percentage of HB cells expressing CD133 after knockdown of CDR1as was determined by flow cytometry. Scale bar, 200 μm. Data are presented as the mean ± SEM of three experiments. *P < 0.05,**P < 0.01,***P < 0.001 (Student’s t-test).