Research Paper Volume 12, Issue 20 pp 20152—20162

PTRF overexpression induced cell cycle arrest at G1 phase and accelerated cellular senescence. (A) Flow cytometry plots showing cell cycle distribution of LSKs from PTRF and WT mice. (B) The percentage of cells in each phase of the cell cycle. (C) Flow cytometry plots showing viable and apoptotic LSKs from PTRF and WT mice. (D) The percentage of apoptotic LSK cells. (E) Flow cytometry plots showing C₁₂FDG levels in the LSKs from PTRF and WT mice. (F) The percentage of SA-β-gal-positive LSK cells. n=4 or 5 mice per group. (G) Flow cytometry plots showing ROS producing cells stained with the DCFH probe. (H) The percentage of ROS-positive LSK cells. Data represent the mean ± SD. *, P < 0.05; **, P < 0.01.