Research Paper Volume 12, Issue 20 pp 20152—20162
Polymerase I and transcript release factor transgenic mice show impaired function of hematopoietic stem cells
PTRF impairs HSCs function. (A) Frequency of colonies generated by LT-HSCs from PTRF and WT mice (n=3). (B) The percentage of CD45.2+ cells in the PB of irradiated recipient mice 4-, 8-, 12- and 16 weeks after transplantation with WT HSCs. (C) The percentage of CD45.2+ cells in the PB of irradiated recipient mice 4-, 8-, 12- and 16 weeks after transplantation with PTRF-overexpressing HSCs. (D, E) The percentage of donor-derived B (D) and M cells (E) from PTRF or WT mice in the BM of recipient mice at 16 weeks post-transplantation. (F) The percentage of donor-derived LSKs in the BM of recipient mice at 16 weeks post-transplantation. n=9 mice per group. Data represent the mean ± SD. *, P < 0.05.