Research Paper
Notch promotes tumor growth and invasion by directly targeting Twist1 in cancer stem cells derived from primary and recurrent glioblastomas
RBPJK binding site in Twist1 promoter is required for molecular interaction with NICD. (A) One possible evolutionary-conserved NICD/RBPJK binding site in Twist1 promotor was revealed using bioinformatic analyses, and mutations of the binding site which are highlighted in red (GG→TT). (B) Measurement of co-transfected firefly luciferase activity in GSCs after co-transfected NICD and wild- or mutant-type Twist1 promoter by bioluminescence assay. The luciferase activity was calculated with an absolute value of the bioluminescent intensity. (C) CHIP assay showed NICD/RBPJK-precipitated fragment binding site. IgG was used as a negative control. Data are shown as the mean ± SD. *: P < 0.05; NS represents no significance.