Research Paper Volume 12, Issue 17 pp 17343—17352
High hsa_circ_0020123 expression indicates poor progression to non-small cell lung cancer by regulating the miR-495/HOXC9 axis
Figure 3. Knockdown of hsa_circ_0020123 suppresses proliferation and invasiveness of NSCLC cells induced by miR-495 sponging. (A, B) qRT-PCR assay of hsa_circ_0020123 expression (A) and miR-495 expression (B) after transfection with sh-hsa_circ_0020123 and miR-495 inhibitor, either singly or in combination. Data are means ± SD. ***P < 0.001 vs. controls. (C, D) CCK8 assay of cell proliferation. Data are means ± SD. ***P < 0.001 vs. controls. #P < 0.05, ##P < 0.01 vs. sh-circRNA. (E, F) Colony formation assay of A549 and H1299 cell proliferation. Data are means ± SD. *P < 0.05, ***P < 0.001 vs. controls. ###P < 0.001 vs. sh-circRNA. (G, H) Transwell assay of A549 cell migration. Data are means ± SD. *P < 0.05, ***P < 0.001 vs. controls. ###P < 0.001 vs. sh-circRNA.