Research Paper Volume 12, Issue 17 pp 17343—17352
High hsa_circ_0020123 expression indicates poor progression to non-small cell lung cancer by regulating the miR-495/HOXC9 axis
Figure 1. hsa_circ_0020123 expression is increased in non-small cell lung cancer (NSCLC) tissues and cell lines. (A) qRT-PCR assay of hsa_circ_0020123 in 20 paired NSCLC tumor tissues and adjacent non-tumor tissues. Data are the means ± SD. ***P < 0.001 vs. normal controls. (B) Fluorescent in situ hybridization (FISH) indicates the subcellular localization of hsa_circ_0020123. (C) The prognostic significance of hsa_circ_0020123 expression for hsa_circ_0020123 patients was performed with FISH values using the median value as the cut-off. The observation time was 60 months. (D) Genomic loci of HOXC9 and hsa_circ_0020123. The red signal indicates back splicing. (E) qRT-PCR assay of hsa_circ_0020123 in A549, PC9, H1299, H1975, H1650, and BEAS-2B normal lung epithelial cells. Data are means ± SD. ***P < 0.001 vs. normal controls. (F) Fluorescent in situ hybridization indicates the subcellular localization of hsa_circ_0020123.