Research Paper Volume 12, Issue 16 pp 16368—16389
Flavonoids from Rosa roxburghii Tratt prevent reactive oxygen species-mediated DNA damage in thymus cells both combined with and without PARP-1 expression after exposure to radiation in vivo
Figure 6. FRT played a role in radiation protection by inhibiting PARP-1 activation and reducing AIF release. (A, B) Expressions of C-PARP-1 and AIF in mouse thymus tissues measured by immunofluorescence. Paraffin-embedded specimens were sliced into sections (4 μm), stained with Cy3 labeled fluorescent secondary antibody (1:500) combined with primary antibody against specific proteins and with DAPI for nuclear staining, sealed with anti-fluorescence quencher, then observed immediately using confocal laser scanning microscopy (n=5).