Research Paper
Exosomal miR-211 derived from cancer stem cells promotes cancer progression by activating autophagy in NSCLC
Figure 5. miR-211 promotes cancer development by inhibiting BCL-W. A549 and H1299 cells were transfected with BCL-W or si- BCL-W and incubated with exosomes from CSCs transfected with miR-211 or AMO-211, respectively. (A, B) Western blot for autophagic associated proteins p62 and LC3I/II in A549 and H1299 cells. n=6, *p<0.05. (C, D) CCK8 was used to test viability of A549 and H1299 cells. n=10, *p<0.05 vs CSC-miR-211+NC or CSC-AMO-211+si-NC. (E, F) Would healing assay to detect migration ability. n=4, *p<0.05 vs CSC-miR-211+NC or CSC-AMO-211+si-NC. (G, H) Transwell assay to detect invasion ability. n=4, *p<0.05 vs CSC-miR-211+NC or CSC-AMO-211+si-NC.