Research Paper
Exosomal miR-211 derived from cancer stem cells promotes cancer progression by activating autophagy in NSCLC
Figure 3. Exo-miR-211 promotes cancer progression by activating autophagy of NSCLC cells. (A) confocal microscope analysis for A549 and H1299 cells transfected with GFP-mRFP LC3. n=10, *p<0.05 vs CSC-miR-NC or CSC-AMO-NC. Scale bar = 100 μM. (B) Western blot for autophagic associated proteins p62 and LC3I/II in A549 and H1299 cells. n=6, *p<0.05 vs CSC-miR-NC or CSC-AMO-NC. A549 and H1299 cells were treated with 3-MA (5 μM) or rapamycin (50 nM). *p<0.05 vs CSC-miR-NC or CSC-AMO-NC. (C–E) Would healing assay to detect migration ability. n=4, *p<0.05 vs CSC-miR-211 or CSC-AMO-211. (F, G) Transwell assay to detect invasion ability. n=4, *p<0.05 vs CSC-miR-211 or CSC-AMO-211.