Research Paper
Exosomal miR-211 derived from cancer stem cells promotes cancer progression by activating autophagy in NSCLC
Figure 2. Exo-miR-211 derived from CSCs accelerate cancer progression of NSCLC cells. A549 and H1299 cells were incubated with exosomes isolated from CSCs transfected miR-211 or AMO-211 or its NC. (A) qRT-PCR analyzed the expression of miR-211 A549 and H1299 cells. n=6, *p<0.05 vs CSC-miR-NC or CSC-AMO-NC. (B) CCK8 was used to test viability of A549 and H1299 cells. n=10, *p<0.05 vs CSC-miR-NC or CSC-AMO-NC. (C, D) Would healing assay to detect migration ability. n=4, *p<0.05 vs CSC-miR-NC or CSC-AMO-NC. (E, F) Transwell assay to detect invasion ability. n=4, *p<0.05 vs CSC-miR-NC or CSC-AMO-NC. (G, H) The number of apoptotic cells was calculated by flow cytometry. n=6, *p<0.05 vs CSC-miR-NC or CSC-AMO-NC.