Research Paper Volume 12, Issue 18 pp 18209—18220

Figure 6. piR-19166 targeted directly EPHA2 in PC3 cells. (A) DNA binding site sequence between piR-19166 and the 3′-UTR of CTTN and the sequence of wild type (WT) or mutant type (MT) were showed. (B) The effect of piR-NC and piR-19166 on the activity of the luciferase reporter containing either WT or MT was detected by dual-luciferase reporter assay. (C, D) RNA and protein levels of CTTN were, respectively, tested by qRT-PCR and western blot in PC3 cells transfected with piR-19166 or anti-piR-19166 compared with those treated with negative control (NC). *P <0.05, **P <0.01.