Research Paper Volume 12, Issue 19 pp 19012—19021
Apremilast ameliorates ox-LDL-induced endothelial dysfunction mediated by KLF6
Apremilast prevented ox-LDL-induced expression of MCP-1 and VCAM-1 and attenuated ox-LDL-induced attachment of U937 monocytes to HUVECs. Cells were treated with ox-LDL (100 μg/mL) in the presence or absence of apremilast (0.5, 1 μM) for 24 h. (A) mRNA of MCP-1 and VCAM-1; (B) Protein of MCP-1 and VCAM-1; (C) Attachment of U937 monocytes to HUVECs was measured by calcein-AM staining (****, P<0.0001 vs. non-treatment group; $$, $$$, P<0.01, 0.001 vs. ox-LDL group, n=4-5).