Research Paper Volume 12, Issue 16 pp 16326—16340
PTEN participates in airway remodeling of asthma by regulating CD38/Ca2+/CREB signaling
Figure 3. PTEN suppresses TNF-α-induced ASM cell proliferation and migration by CD38. ASM cells were infected with Ad-PTEN and/or Ad-CD38 and incubated for 48 h, which was followed by stimulation with o20 ng × ml-1 TNF-α for 24 h, and (A) protein expression of PTEN and CD38 was examined via Western blot. Quantitative analysis of the expression of the (B) PTEN and (C) CD38 protein determined in A (n = 3, **p < 0.01, ***p < 0.001, ****p < 0.0001). (D, E) Cell proliferation assessed using an EdU proliferation assay (magnification: 200X) (n = 3, *p < 0.05, **p < 0.01, ***p < 0.001,). (F, G) Cell migration evaluated using a Transwell migration assay (magnification: 200X) (n = 3, **p < 0.01, ***p < 0.001, ****p < 0.0001).