Research Paper Volume 12, Issue 17 pp 17122—17136

Figure 3. Overexpressed IL-6R in SKOV3 response to miR-21/IL-6 crosstalk elevates expression of PD-L1. (A, B) The expression of IL-6R and PD-L1 in ovarian cancer biopsy specimens analyzed by IHC. (C) correlation between IL-6R and PD-L1. (D) knock down efficiency of IL-6R assessed by western blotting. (E) expression of miR-21 after depletion of IL-6R determined by RT-qPCR. (F) change of PD-L1 after IL-6R silencing assessed by Western blotting analysis. (G) proliferation of T cells after inhibition of IL-6R analyzed by flow cytometry. * p < 0.05 vs. control or normal. Statistical comparisons were performed using unpaired t test when only two groups were compared or by Tukey’s test-corrected one-way ANOVA with when more than two groups were compared.