Research Paper Volume 12, Issue 16 pp 16255—16269

Figure 2. The effects of glucose, trehalose, cholesterol, and Δ⁷-dafachronic acid on the survival of L1-arrested worms. Glucose extends the longevity of L1-arrested ins-6 (oe) (A) and daf-18 mutants (B). Trehalose extends the longevity of L1-arrested ins-6 (oe) (C) and daf-18 mutants (D). (E) Trehalose has no suppression function on Q-cell proliferation; in contrast, it can induce Q-cell proliferation in wild-type L1-arrested worms. Data are the average of at least three independent experiments. Error bars: Standard Deviation (SD).***: P<0.001. (F) The glycogen synthesis controlling gene gsy-1 has no effect on the survival of L1-arrested worms. (G) The glycogen and trehalose synthesis controlling genes gsy-1 and tps-1/2 have no effect on the Q-cell divisions in daf-18 (-), ins-6 (oe), and wild-type L1-arrested worms. (H) Cholesterol and dafchronic acid can extend the survival of wild-type L1-arrested worms. Survival of these worms was checked every day, and the mean survival rate was calculated using the Kaplan-Meier method, and any significant difference in overall survival rates was determined using the log-rank test (P-value; see Supplementary Table 1).