Mst1 promotes mitochondrial dysfunction and apoptosis in oxidative stress-induced rheumatoid arthritis synoviocytes

Yingjie Wang; Qi Yang; Songpo Shen; Linjie Zhang; Yongbo Xiang; Xisheng Weng

doi:doi:10.18632/aging.103643

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Research Paper Volume 12, Issue 16 pp 16211—16223

Mst1 promotes mitochondrial dysfunction and apoptosis in oxidative stress-induced rheumatoid arthritis synoviocytes

Figure 3. Mst1-dependent Sirt1 downregulation in oxidative stress-induced RA-FLSs. (A) QRT-PCR assay shows Sirt1 mRNA levels in control and H₂O₂-treated RA-FLSs. (B, C) Immunofluorescence staining shows Sirt1 protein levels in the control and Mst1-silenced RA-FLSs treated with or without H₂O₂. (D, E) QRT-PCR assay shows Sirt3 and Sirt6 mRNA levels in the control and H₂O₂-treated RA-FLSs. Note: RA-FLSs were treated with 0.3 mM H₂O₂ for 6 h; *P<0.05.