Research Paper Volume 12, Issue 20 pp 20127—20138

Figure 6. DLEU1 knockdown reduces in vivo growth of xenograft tumors in nude mice model. (A) The curve shows the rate of growth of xenograft tumors generated from subcutaneously injected control and DLEU1 knockdown TPC-1 cells in the nude mice (n=5 each). Tumor growth was measured every 7 days for 28 days. (B) The representative images show the xenograft tumors in nude mice that are subcutaneously injected with control and DLEU1 knockdown TPC-1 cells for 28 days. (C) The histogram plot shows the weight of xenograft tumors derived from nude mice subcutaneously injected with control and DLEU1 knockdown TPC-1 cells. (D) Representative images show IHC staining with the anti-Ki67 antibody of xenograft tumor tissue sections derived from nude mice subcutaneously injected with control and DLEU1 knockdown TPC-1 cells. (E–F) QRT-PCR analysis shows the levels of DLEU1 and miR-421 in the xenograft tumor tissues derived from nude mice subcutaneously injected with control and DLEU1 knockdown TPC-1 cells. (G) QRT-PCR analysis shows ROCK1 mRNA levels in the xenograft tumor tissues derived from nude mice subcutaneously injected with control and DLEU1 knockdown TPC-1 cells. (H) Western blot analysis shows ROCK1 protein levels in the xenograft tumor tissues derived from nude mice subcutaneously injected with control and DLEU1 knockdown TPC-1 cells. Note: The data are represented as the means ± SD of at least three independent experiments. *P< 0.05 and **P< 0.01.