Research Paper Volume 12, Issue 17 pp 17099—17113

Figure 6. Deacetylation activity of SIRT6 on H3K9ac, H3K18ac and H3K56c is not required for spermatogenesis. (A) The levels of H3K9ac in Sirt6^+/+ and Sirt6^-/- testes were detected by western blot. 8-week mice, n=3. (B) Localization of H3K9ac in Sirt6^+/+ and Sirt6^-/- seminiferous tubules. Testes sections of Sirt6^+/+ and Sirt6^-/- stained with SYCP3 (green) and H3K9ac (red) antibodies. 8-week mice, n=4. (C) Quantification of H3K9ac intensity of round spermatids and elongated spermatids in the Sirt6^+/+ and Sirt6^-/- mice. Sirt6^+/+, 15.82±0.48; Sirt6^-/-, 14.78±0.58. 8-week mice, n=4; 200 cells were used for each group. Data are presented as mean ± SEM. (D) The levels of H3K18ac in Sirt6^+/+ and Sirt6^-/- testes were detected by western blot. 8-week mice, n=3. (E) The levels of H3K56ac in Sirt6^+/+ and Sirt6^-/- testes were detected by western blot. 8-week mice, n=3. (F) A functional diagram of Sirt6 in spermatogenesis.