Figure 4. DAPK1 protein expression is reduced in advanced ccRCC patient tissues. (A, B) Western blotting and grayscale analysis results show DAPK1 protein expression in 8 pairs of ccRCC and adjacent normal kidney tissue samples. GAPDH is used as internal control. (C) Immunohistochemical (IHC) analysis shows DAPK1 protein expression in ccRCC tissues from patients with different Fuhrman stages. (D) IHC analysis shows DAPK1 protein expression in normal and ccRCC tissues based on IHC analysis in The Human Protein Atlas database.