Research Paper Volume 13, Issue 10 pp 14469—14481

Figure 5. PXN enhances tumor formation ability, cell proliferation and metastasis, but suppresses cell apoptosis in gastric cancer. (A) Relative expression of PXN in gastric cancer tissues (n = 65) and the adjacent normal tissues determined by RT-qPCR; *, p < 0.05 compared with adjacent normal tissues; (B) Relative expression of PXN in gastric cancer tissues from patients with metastasis (n = 45) and without metastasis (n = 20) determined by RT-qPCR; *, p < 0.05 compared with gastric cancer tissues from patients without metastasis; In the panels (C–F), SGC7901 cells were transfected with oe-PXN or co-transfected with oe-PXN and si-XIST. (C) The mRNA expression of PXN detected by RT-qPCR; (D) Cell proliferation ability in each group detected by EdU assay (× 400); (E) Cell migration ability detected by scratch test (× 40); (F) Cell apoptosis ability detected by flow cytometry; (G) The effect of PXN and lncRNA XIST on tumor formation in nude mice injected with the stably transfected SGC7901 cells. All measurement data and statistical results were expressed as mean ± standard deviation. *, p < 0.05 compared with cells transfected with oe-NC or the mice injected with the oe-NC-transfected cells. Comparisons among multiple groups were analyzed using one-way ANOVA. The experiment was repeated 3 times.