Research Paper Volume 12, Issue 18 pp 18127—18136

Figure 4. GHR knockdown enhanced sorafenib promoting of cell apoptosis and blocking of cell cycle progression. (A) Caspase-3 activity assay detected caspase-3 activity in HCC cell lines with siGHR or/and sorafenib; (B) The results of western blotting assay showed that the protein levels of cleaved caspase-3 were significantly increased in HCC cell lines with siGHR and sorafenib combination compared with cells with sorafenib single; (C) GHR inhibition combined with sorafenib prevented HCC cell progression from G stage to S and G2M stages. All data shown represented the mean ± SEM. ***P < 0.001, compared with control groups.