Research Paper Volume 12, Issue 15 pp 15414—15435
Depletion of exosomal circLDLR in follicle fluid derepresses miR-1294 function and inhibits estradiol production via CYP19A1 in polycystic ovary syndrome
Figure 4. Validation of the RNA sequencing results by qRT-PCR. Five differentially expressed circRNAs (chr17:16075117-16097953-/ hsa_circ_0005069, chr19:11230768-11231198+/ hsa_circ_0006877, chr15:68352856-68353111+, chrM:2262-2426- and chr13:110076504-110076682+) were validated by qRT-PCR. The red bars show the relative gene expression measured using qRT-PCR. The blue bars show the relative gene expression measured using RNA sequencing. N=5 vs 5 for RNA sequencing, and N=25 vs 25 for qRT-PCR.