Research Paper Volume 12, Issue 16 pp 16172—16182
Leptin promotes bone metastasis of breast cancer by activating the SDF-1/CXCR4 axis
Figure 3. Leptin receptor mediated, leptin-induced EMT in breast cancer cells. (A–C) Real-time PCR was performed to examine Vimentin, E-cadherin, and CXCR4 expression in MCF-7 and SK-BR-3 cells. Data are shown as the means ± SD. *P<0.05, **P<0.01, ***P<0.001. (D) Western blot was employed to detect Vimentin, E-cadherin, and CXCR4 expression in MCF-7 cells. (E–G) Real-time PCR was used to detect Vimentin, E-cadherin, and CXCR4 expression in MCF-7 and SK-BR-3 cells. Data are shown as means ± SD. ns, not significant.