Research Paper Volume 12, Issue 16 pp 16172—16182

Figure 2. Leptin promoted breast cancer cell metastasis and leptin receptor expression. (A) Western blot was used to detect Ob-Rb and Ob-Rt expression in MCF-7 and SK-BR-3 cells. (B, C) The effect of leptin on invasion of MCF-7 and SK-BR-3 cells was examined using the transwell assay. The upper and lower chambers of the wells were separated by polycarbonate membranes. Breast cancer cells (5×10⁴/well) were seeded in the upper chambers of the 24-well plate with serum-free medium. The lower chamber was filled with medium containing 5% fetal bovine serum as a chemoattractant. The data are shown as means ± SD. **P<0.05. (D) Western blot was used to detect leptin receptor expression in MCF-7 and SK-BR-3 cells.