Research Paper Volume 12, Issue 15 pp 15374—15391

Figure 4. Oncogenic function of SCD1 in gastric cancer cells. (A–C) Gastric cancer cells were treated with 1 μM Erastin for 24 hours, then cell death was assessed using propidium iodide (PI), the bar plot represent quantification of PI-positive cells. Each experiment was conducted in triplicates. (D–F) C11-BODIPY staining of gastric cancer cells following treatment with 1 μM Erastin for 24 hours, the bar plot represent quantification of C11-BODIPY-positive cells. Each experiment was conducted in triplicates. (G, H) SCD1 ameliorated the migration ability of gastric cancer cells. (I, J) The metastatic related markers were analyzed by using western blotting with the indicated antibodies. (K, L) the cancer stemness related markers were analyzed by using western blotting with the indicated antibodies. GAPDH was used as the internal protein loading control. Each experiment was conducted in triplicates. *, P <0.05; **, P <0.01; ***, P <0.001; ****, P <0.0001, respectively.