Research Paper Volume 12, Issue 18 pp 18052—18072

Figure 5. Exogenous miR-21 was delivered by EVs into recipient tubular cells and promoted EMT. (A) Western blot analysis showed that resveratrol inhibited senescence of recipient cells, blocking EVs from high-glucose conditioned medium induced decline of E-cadherin and increase of α-SMA and vimentin. (C) Western blot analysis showed that inhibition of miR-21 by transfection of miR-21 inhibitor in recipient cells blocked EVs from high-glucose conditioned medium induced decline of E-cadherin and increase of α-SMA and vimentin. Upregulation of miR-21 by miR-21 mimics transfection in recipient cells promoted EVs-induced inhibition of E-cadherin and inductions of α-SMA and vimentin expression. (B, D) Quantitative analysis of band density for the proteins. The protein expression data are presented as the mean ± SD. * p < 0.05 (HG vs CON); ^Δp < 0.05 (HG vs HG+inhibitor). ^# p < 0.05 (mimics vs CON); **/^ΔΔ/^## p < 0.01.