Research Paper Volume 12, Issue 16 pp 16142—16154
LAIR-1 suppresses cell growth of ovarian cancer cell via the PI3K-AKT-mTOR pathway
Figure 4. LAIR-1 suppresses ovarian cancer cell growth by regulating PI3K-AKT-mTOR signal axis. (A) Western blot analyses of PI3K, AKT, p-AKT, mTOR, p-mTOR, P70S6K, p-P70S6K, 4E-BP1 and p-4E-BP1 levels in control and LAIR-1 overexpression SKOV3 cells, control and LAIR-1 knockdown (shRNA) HO8910 cells. (B, C) These proteins were quantified according to the gray value of each band. And the data expressed in right graphs represent the mean ± SD in both SKOV3 and HO8910 cells. (D) The control and LAIR-1 knockdown HO8910 cells were treated with or without PI3K inhibitor LY294002, and then the cell viabilities was detected by CCK-8 assay. Cell growth curves were plotted according to the OD values. Data are presented as the mean ± SD (**p < 0.01, ##p < 0.01).