Research Paper Volume 12, Issue 18 pp 18019—18032
Long non-coding RNA AGAP2-AS1 increases the invasiveness of papillary thyroid cancer
Figure 3. AGAP2-AS1 suppression decreases MMP2 expression by upregulating miRNA-424-5p in PTC cells. (A) FISH analysis demonstrating cytoplasmic localization of AGAP2-AS1 in TPC1 and K1 cells. (B) qRT-PCR of miRNAs in TPC1 and K1 cells after AGAP2-AS1 knockdown. Data are presented as the mean ± S.D, analyzed using an independent samples t-test; **P < 0.01 vs. NC. (C, D) Western blotting of MMP2 in TPC1 and K1 cells incubated with miR-424-5p mimic. Data are presented as the mean ± S.D, analyzed using an independent samples t-test; *P < 0.05, **P < 0.01 vs. NC.