Research Paper Volume 12, Issue 14 pp 15021—15036
NVD-BM-mediated genetic biosensor triggers accumulation of 7-dehydrocholesterol and inhibits melanoma via Akt1/NF-ĸB signaling
Figure 8. NVD-BM functions as a tumor suppressor via Akt1/NF-κB signaling. (A) GSEA revealed up-regulated genes associated with NF-κB signaling in melanoma (GSE3189 dataset). (B) Gene expression of the NF-κB target genes, MMP9, CCL20 and IL1B relative to GAPDH, in A375 cells transfected with NVD-BM, when compared with A375 cells transfected with blank vector, pCDH. (C) Western blot of A375 cells transfected with two different plasmid NVD-BM concentrations, when compared with pCDH blank vectors, analyzing the entry of RELA into the nucleus. (D) Western blot of A375 cells transfected with two different plasmid NVD-BM concentrations, when compared to the pCDH blank vector analyzed phosphorylated and unphosphorylated Akt1-SER473 with normalization to GAPDH. (*p < 0.05, **p < 0.01, ***p < 0.001).