Research Paper Volume 12, Issue 14 pp 15011—15020
Circular RNA hsa_circ_0076690 acts as a prognostic biomarker in osteoporosis and regulates osteogenic differentiation of hBMSCs via sponging miR-152
Figure 4. (A) The expression of hsa_circ_0076690, miR-152 and RUNX2 were measured by qRT-PCR during osteogenic differentiation of hBMSCs. (B) MiR-152 and RUNX2 expression levels were measured by qRT-PCR after transfected with hsa_circ_0076690 in hBMSCs. (C) RUNX2 expression was inhibited by miR-152 mimics and remained constant under miR-152/hsa_circ_0076690 co-overexpression. (D) The expression level of osteogenic-associated biomarkers and ALP activity were detected after transfected with hsa_circ_0076690, miR-152 mimics and miR-152/hsa_circ_0076690 co-overexpression in hBMSCs.