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Research Paper Volume 12, Issue 23 pp 23598—23608
Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway
Figure 4. Exosomal-Hic-5 effected the proliferation and apoptosis of OS. (A) Immunofluorescence assays was performed to detect co-cultured results in OS cells. (B) MTT assay was performed to detected cell viability in co-cultured OS cells. n=10, *P<0.05. (C) The proliferation ability of co-cultured OS cells was measured by Edu assay. (green, Edu+, blue, DAPI). n=6, *P<0.05. (D) The apoptosis rate was detected by flow cytometry. n=5, *P<0.05. (E) The caspase3 activity was assessed in co-cultured OS cells. n=6, *P<0.05. (F) The apoptosis-associated protein (Bcl2, Bax and Cleaved-caspase3) level was detected by western blot. n=6, *P<0.05. (G) The size of exosomes co-cultured MG-63 xenograft tumors in nude mice. (H) The tumor volume and weights of removed tumor were calculated. n=15, *P<0.05. (I) Representative Ki-67 staining of tumor sections in different group.