Research Paper Volume 12, Issue 18 pp 17990—18007

Figure 3. Activation of SIRT1 induces HO-1 upregulation in microglial cells. (A) BV-2 microglia were stimulated with the SIRT1 activator, CAY (5 μM) or SRT (1 μM), for the indicated time periods. (B) IMG cells were stimulated with the SIRT1 activator, CAY (5 μM), for the indicated time periods. (C) IMG cells were transfected with empty vector or wild-type SIRT1 for 24 h. (D) BV-2 (left panel) and IMG (right panel) microglial cells were treated with SIRT1 activator CAY (5 μM), LPS (100 ng·mL⁻¹), or both CAY and LPS for 24 h. Whole-cell lysate proteins were extracted, and the HO-1 protein levels were determined by western blot analysis. (E) After transfection of BV-2 microglia with siRNA against SIRT1 or control for 24 h, the cells were treated with the SIRT1 activator for another 24 h. HO-1 and SIRT1 expression levels were analyzed by western blot analysis. Similar results were obtained from three independent experiments.