Research Paper Volume 12, Issue 14 pp 14791—14807

Figure 1. Identification of miR-185 targeting the 3’-UTR of shelterin POT1. (A) Transient dual luciferase reporter assay of HEK293T cells following expressing each candidate miRNA and the POT1 3’-UTR. P values were determined by Student’s t-test. *P<0.05, **P<0.01, ***P<0.001. (B) List of significant candidate miRNAs and the relative luciferase activities of cells from (A). (C) Relative POT1 mRNA level after overexpression of each candidate miRNA in HTC75 cells. P values were determined by Student’s t-test. **P<0.01, ***P<0.001. (D) Venn diagram of significant candidate miRNAs that downregulate either POT1 3’-UTR luciferase activity or endogenous POT1 mRNA levels. (E) The predicted seed region of the miR-185 target sites was mutated (in bold). The ability of miR-185 to target the seed regions of the wild-type (wPOT1) vs. mutant (mPOT1) 3’-UTR was determined by dual luciferase assay. P values were determined by Student’s t-test. **P<0.01, ***P<0.001.