Expression of Concern
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This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.
Research Paper Volume 12, Issue 14 pp 14775—14790
The circular RNA circEIF3M promotes breast cancer progression by promoting cyclin D1 expression
Figure 9. CCND1 is directly targeted by miR-33a. (A) Schematic illustration of CCND1 3’UTR-WT and CCND1 3’UTR-Mut luciferase reporter vectors. (B, C and E) Relative mRNA and protein levels of CCND1 were detected in cells after transfected with miR-NC, miR-33a, NC inhibitor and miR-33a inhibitor using qRT-PCR, respectively. (D) The relative luciferase activities were detected after transfected with CCND1 3’UTR-WT or CCND1 3’UTR-Mut and miR-33a mimics or miR-NC, respectively. (F and G) Relative expression of CCND1 was detected by qRT-PCR in cells transfected with indicated vectors, circEIF3M or sh-circEIF3M. Data were indicated as mean ± SD, **P < 0.01.