Research Paper Volume 12, Issue 17 pp 16837—16851
Sulforaphane-cysteine downregulates CDK4 /CDK6 and inhibits tubulin polymerization contributing to cell cycle arrest and apoptosis in human glioblastoma cells
Figure 4. SFN-Cys decreased the expressions of CDK4, CDK6 and p-Rb via sustained ERK1/2 phosphorylation. (A–D) Both U87MG and U373MG cells were pretreated with PD98059 (25 μM) for 30 min, then treated with SFN-Cys (30 μM) for 24 h. The expressions of CDK4, CDK6, p-Rb, Rb and pERK1/2 were analyzed by Western blot. Data were shown as means ± SD (n ≥ 3). *, P ≤ 0.01.