Research Paper Volume 12, Issue 14 pp 14699—14717

Figure 3. HSD17B4 promotes the migration of PCa cells. (A–B) Knockdown of HSD17B4 inhibits the migration of PCa cells, while HSD17B4 overexpression promotes cell migration. LNCaP, DU145 and PC3 stable cells were treated as indicated and analyzed by migration assays in 24-well chambers without Matrigel. Quantitative analysis of cell migration was performed by ImageJ. (C) Knockdown of HSD17B4 inhibits migration of PCa cells, while HSD17B4 overexpression promoted cell migration. LNCaP, DU145 and PC3 stable cells treated as indicated were analyzed by a wound-healing assay. Scale bars: 200 μm. Quantitative analysis of the wound healing area was performed by ImageJ. (D) Knockdown of HSD17B4 increases CDH1 but decreases CDH2 and VIM in LNCaP cells, while HSD17B4 overexpression induces the inverse results (left panel). Protein levels of CDH1, CDH2, VIM and HSD17B4 were normalized against ACTB, *denotes P<0.05, ^**denotes P < 0.01. Error bars represent ±SD of triplicate experiments (right panel). Data are shown as the mean ± SD (n = 3) or typical photographs of one representative experiment. Similar results were obtained in three independent experiments. *p < 0.05, **p < 0.01, ***p <0.001.