Research Paper Volume 12, Issue 14 pp 14391—14405

Figure 3. Loss of DDR1 inhibits EMT and glutamine metabolism in SNU-182 cells. The siRNA of DDR1 or NC was transfected into SNU-182 cells. The expression of DDR1 was detected by qRT-PCR (A) and western blot (B) (n = 6, *p<0.05). (C) CKK-8 assay was used to examine cell growth at 0, 24, 48 and 72 h (n = 10, *p<0.05). (D) Wound healing assay was used to detect cell migration (n = 6, *p<0.05). (E) Transwell assay was performed to check cell invasion (n = 6, *p<0.05). qRT-PCR (F) and western blot (G) were used to test the expression of EMT related genes: Vimentin, N-cadherin and E-cadherin (n = 6, *p<0.05). (H) The protein expression of glutamine metabolism related genes: GLUD1, GLS1 and SLC1A was determined by western blot (n = 6, *p<0.05).